Several Methods of the Centrifugation of High Speed Centrifuge

1. Brief introduction of the high speed centrifuge


The high speed centrifuge gets its name because of its high speed, and its performance and structure are actually similar to traditional centrifuge machines. Because of the high speed, the requirements of the rotor are higher than those of ordinary ones. Especially in terms of material, more titanium alloys and aluminum alloys are used. Also the centrifuge tube is also made of a specific material, which is a polyethylene hard plastic product with a cover. This type of centrifuge machine is mostly used to collect microorganisms, cell debris, cells, large organelles, sulfuric acid precipitates, and immunoprecipitates.


2. Several centrifugation methods of the high speed centrifuge


1) Differential centrifugation:


The centrifugal force is gradually increased, and some components in the sample solution can be settled each time.


Differential centrifugation is the most commonly used method. In this method, the high speed centrifuge tube is initially filled with a uniform sample solution. After centrifugation at a certain speed for a certain period of time, two parts can be obtained: precipitation and supernatant.


Usually during the first centrifugation, most of the unneeded large particles are settled and removed. At this time, most of the required components remain in the supernatant. The collected supernatant is then centrifuged at a higher speed to deposit the desired particles. The centrifugation time should be chosen appropriately so that most of the smaller particles that are not needed remain in the supernatant. The obtained precipitate and supernatant can be further centrifuged until the required separation purity is achieved. The characteristic of differential centrifugation is simple operation, but the separation purity is not high.


2) Density gradient centrifugation: 


It can separate several or all components in the sample at the same time, with good resolution.


3) Isopycnic centrifugation (isopycnic)

The method is to achieve centrifugation according to the different density of the particles. During centrifugation, the particle will move to a place with the same density as itself to form a zone.

  

The density sample should be selected so that the range of the gradient includes the density of all particles to be separated. The sample can be on top of the density gradient liquid particles or evenly distributed in the density gradient. After centrifugation, the sample particles reach their equilibrium point. Note: The centrifugation of particles after equilibrium is completely determined by their density and has nothing to do with time. At this time, changing the centrifugal speed can only change the relative position of the zone.


Finally, ZONKIA wants to say that no matter how good the instrument is, the daily correct operation and daily maintenance are inseparable. Once a failure occurs, it will delay the experiment and it will not be worth the loss! Safety is even more important when using a centrifuge machine. Uncontrollable centrifugal force may cause great damage. Therefore, pay attention to whether the centrifuge tube is balanced, whether the speed exceeds the setting, whether the rotor is corroded, etc.


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